Ricerca, Progetti SafeCrop

ARMILLARIA:

Researcher: Michele Perazzolli

Partner(s) and place of activities: SafeCrop Centre IASMA 

Scientific responsible of the project: Dr. Claudio Moser (Genetics and Molecular Biology Dep.), Dr. Cesare Gessler (SafeCrop)

SUMMARY or LIST of activities planned in 2006: 

Pseudomonas fluorescens transformation with grape genes induced during Armillaria mellea infection: characterization of the biological control properties and the effects on the rhizosphere

• Bioinformatic analyses of the grape genes induced in Kober 5BB roots during A. mellea infection
• Validation of defence gene induction in different infection systems (in vivo and in vitro)
• Amplification, cloning and sequencing of the full-length coding sequences of the genes
• Genes subcloning in a vector suitable for the expression in E. coli and characterization of antifungal activity of the recombinant proteins
• Cloning of the most effective defence genes in a vector suitable for expression in Pseudomonas spp., transformation of P. fluorescens and in vitro characterization of the biological control properties of the transgenic P. fluorescens
• Characterizations of the transgenic P. fluorescens distribution in the soil and its impact on the rhizosphere microorganisms equilibrium (if time left)

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